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Melanoma in HPB

Melanoma in Hepato-Pancreato-Biliary (HPB) Diseases

Introduction

  • Melanoma is a malignant tumor of melanocytes, known for its aggressive behavior and potential to metastasize to various organs.
  • Gastrointestinal metastases of malignant melanoma are common; however, isolated disease of the pancreas is rare, with surgical experience limited to small case series.

Melanoma Metastases to the Pancreas

Incidence and Clinical Features

  • Pancreatic metastases from melanoma are uncommon but have been reported, often originating disproportionately from primary ocular melanoma.
  • In up to 13% of cases, the primary site may not be identified, suggesting a possibility of misdiagnosis as a primary pancreatic malignancy.

Surgical Management

  • Surgical resection for pancreatic metastases from melanoma should be considered a palliative treatment.
  • Indications for resection:
    • Patients with long disease-free intervals after treatment of the primary tumor.
    • Negative extent-of-disease workup indicating isolated pancreatic involvement.
    • After a multidisciplinary discussion.
  • Prognosis after resection:
    • Small series suggest a 5-year survival of 50% for patients with R0 resected isolated melanoma metastases.
    • In comparison, a 9% 5-year survival for patients treated with non-operative management.
  • Due to retrospective and highly selective data, a clear benefit of resection cannot be firmly established.

Imaging Findings

  • Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) play crucial roles in detecting pancreatic metastases.
  • Hypervascular neoplasms like melanoma are generally evaluated using a multiphasic protocol that includes:
    • Precontrast imaging
    • Late arterial phase
    • Portal venous phase
  • FDG-PET scans may be useful, especially if the patient has a history of colonic, renal, or melanoma primary tumors.

Familial Atypical Multiple Mole and Melanoma (FAMMM) Syndrome

Genetic Basis

  • FAMMM syndrome is associated with p16 germline mutations, a tumor suppressor gene that may be mutated or have altered expression due to post-transcriptional methylation.

Clinical Features

  • Predisposes individuals to:
    • Melanomas
    • Multiple nevi
    • Atypical nevi
    • Pancreatic cancer (PC)
  • Patients with FAMMM have:
    • An approximately 80% lifetime risk of melanoma.
    • A 20% to 34-fold increased risk of pancreatic adenocarcinoma (PDA) over their lifetime.

Melanoma Metastases to the Liver

Incidence and Clinical Features

  • Metastatic melanoma to the liver can occur, sometimes with an unknown primary site.
  • Melanoma metastases can form polypoid lesions and mimic primary tumors, especially when the history of melanoma is not apparent.

Imaging Findings

  • CT Imaging:
    • Melanoma liver metastases are hypervascular lesions.
    • Multiphasic imaging is advantageous for detecting hypervascular liver metastases.
    • Approximately 14% of melanoma metastases are not seen on portal venous phase images alone.
  • MRI Imaging:
    • Metastases may be hyperintense on T1-weighted images due to melanin content.
    • On T2-weighted images, they are usually moderately brighter than the liver.
    • Diffusion-weighted imaging (DWI) can provide additional signal characteristics.
    • Subtraction imaging can demonstrate the "doughnut sign", indicating peripheral rim enhancement and central hypoenhancement due to necrosis.

Surgical Management

  • Some propose that patients with isolated hepatic melanoma metastases may benefit from surgery when complete resection is possible.
  • Outcomes after resection:
    • Reports suggest improved survival rates in selected patients.
    • Resection for non-colorectal, non-neuroendocrine (NCNN) liver metastases shows varying survival benefits based on the primary tumor site.
    • Melanoma liver metastases showed promising survival outcomes in some studies.

Melanoma Involving the Biliary Tree

Metastases Mimicking Primary Tumors

  • The biliary tree can be involved by metastases from various carcinomas, including melanoma, which may form polypoid luminal lesions.
  • Metastatic melanoma can mimic primary biliary tumors, often without an apparent history of melanoma.

Imaging and Pathology

  • Gross appearance:
    • Metastatic melanoma in the pancreas or biliary tree may appear black if intensely pigmented.
  • Microscopic examination:
    • Melanoma cells are not arranged in a particular pattern and display diffuse, noncohesive infiltration.
    • Immunohistochemistry:
      • Positive for melanocytic markers (S100, SOX10, Melan-A, HMB45).
      • Negative for cytokeratin, helping to confirm the diagnosis.

Molecular Aspects

  • Mutational burden in HPB malignancies is not exceptionally high compared to other cancers.
  • Melanomas fall at the upper end of the spectrum with higher mutational loads.
  • Tumor Mutation Burden (TMB):
    • Positively correlated with increased likelihood of neoantigen production.
    • Potentially results in a beneficial response to checkpoint inhibitor therapies.

Treatment

Role of Type I Interferons

  • Type I Interferons (IFNs), including Interferon-α (IFN-α) and Interferon-β (IFN-β), are crucial in the immune response to viral infections.
  • Clinical use:
    • IFN-α has been used in the treatment of viral hepatitis and as an adjuvant therapy for melanoma.
  • Mechanism of action:
    • Direct antiviral action by triggering infected cells to produce enzymes that interfere with viral replication.
    • Increase expression of MHC class I molecules, enhancing recognition by CD8+ T cells.
    • Inhibit cellular proliferation.

Chemotherapy and Immunotherapy

  • The efficacy of surgical treatment compared with evolving forms of chemotherapy and immunotherapy is not well-established due to insufficient data.
  • Checkpoint inhibitors and other immunotherapies may be considered, especially given the higher TMB in melanoma.

Conclusion

  • Melanoma metastases to the pancreas, liver, and biliary tree are rare but clinically significant.
  • Surgical resection may offer survival benefits in selected patients with isolated metastases and long disease-free intervals.
  • FAMMM syndrome significantly increases the risk of melanoma and pancreatic cancer, necessitating careful monitoring.
  • Imaging techniques like CT, MRI, and FDG-PET are essential for detection and management planning.
  • Molecular profiling and understanding of TMB are important for guiding immunotherapy options.
  • A multidisciplinary approach is crucial in managing melanoma in HPB diseases to optimize patient outcomes.


Melanoma in Hepato-Pancreato-Biliary (HPB) Diseases

Introduction

  • Melanoma is a malignant tumor of melanocytes known for its aggressive behavior and potential to metastasize to various organs.
  • In 2020, melanoma was estimated to account for approximately 7% of new cancer cases in men and 5% in women in the United States.
  • Most cases (90%) involve melanoma of cutaneous origin, whereas a small subset is derived from the uvea (5%) or other body sites.
  • The pattern of metastatic spread depends on the site of the primary melanoma.

Cutaneous Melanoma

Incidence and Patterns of Metastasis

  • Cutaneous melanomas disseminate to the liver in 15% to 20% of patients with metastatic disease.
  • They are often associated with simultaneous extrahepatic disease.
  • Cutaneous melanoma recurs after potentially curative resection in approximately one-third of patients, with almost every organ being at risk.
  • Hepatic metastases are diagnosed in approximately 10% to 20% of patients with stage IV melanoma, even though it is well documented that most patients have liver metastases on postmortem examination.

Surgical Management

  • Before the introduction of targeted therapy (BRAF and MEK inhibitors) and checkpoint inhibitors, most patients were considered to have unresectable melanoma due to extrahepatic disease or disseminated hepatic metastases.
  • Study from John Wayne Cancer Institute and Sydney Melanoma Unit (1971-1999):
    • 26,204 patients with melanoma were reviewed.
    • 1,750 patients (6.7%) had liver metastases.
    • Only 34 patients underwent surgical exploration for attempted liver resection.
      • Hepatectomy was performed in 24 patients.
      • Of these, 12 had synchronous extrahepatic disease, and 18 could be rendered disease-free surgically.
    • 10 patients who underwent exploration only had a median survival of 4 months.
    • The overall survival of all patients with liver metastases treated nonoperatively was 6 months.
    • Patients who underwent complete resection had an overall survival of 28 months, with a median disease-free survival (DFS) of 12 months.
      • One patient achieved 10-year survival, and the 5-year overall survival was 29%.
    • Complete gross resection and histologically negative margins were associated with improved DFS.
      • Complete gross resection showed a trend for improved overall survival (P = .06).
  • Conclusions:
    • Extrahepatic disease per se is not a contraindication for resection, provided that all disease is resectable.
    • Patient selection is crucial.
      • Only 1.4% of patients with liver metastases underwent hepatic resection.
      • These patients had a median disease-free interval of 58 months before presenting with hepatic metastases.
  • Other small series report median survival times of 10 to 51 months after liver resection for metastatic melanoma.
  • Repeat hepatic resection might be beneficial for some patients who can be rendered disease-free surgically.

Impact of Targeted Therapies

  • In the last decade, there has been a paradigm shift in the treatment of metastatic melanoma with the introduction of targeted therapy using BRAF or MEK inhibitors and immune checkpoint inhibitors.
  • Current studies have shown that the median survival in selected patients with metastatic melanoma and a BRAF V600 mutation can be extended to more than 21 months.
  • Combination therapy with dabrafenib and trametinib resulted in an objective tumor response rate of 64%.
  • This effective systemic therapy has a powerful impact on patients undergoing resection in metastasized melanoma.
  • Recent studies have shown that patients undergoing hepatectomy for liver metastases of melanoma can experience:
    • 1-year survival of 51%.
    • 2-year survival of 38%.
  • These data are encouraging in that targeted therapy might render more patients eligible for a curative surgical approach when they present with liver metastases.

Uveal Melanoma

Incidence and Patterns of Metastasis

  • Uveal melanoma is a distinct entity with different tumor biology and commonly spreads to the liver.
  • Approximately 50% to 80% of patients with uveal melanoma who develop distant metastases have liver involvement.
  • In 40% of patients with liver metastases from uveal melanoma, the liver is the only organ of secondary disease without additional extrahepatic disease.
  • Therefore, the number of liver metastases resected from uveal melanoma is nearly equivalent to that of cutaneous melanoma.

Surgical Management

  • Mariani et al. investigated the management of liver metastases from uveal melanoma in 798 patients.
    • 255 patients received surgical treatment.
    • The median overall survival after hepatic surgery was 14 months compared with 8 months in those who had no surgery.
    • Survival analysis based on resection status showed:
      • R0 resection: median overall survival of 27 months.
      • R1 resection: median overall survival of 17 months.
      • R2 resection: median overall survival of 11 months.
    • Prognostic classifiers identified were:
      • Time to liver metastases ≤24 months.
      • Number of liver metastases resected ≤4 lesions.
      • Absence of miliary disease.
  • Hsueh et al. reported on 112 patients with metastatic uveal melanoma.
    • 78 patients had liver metastases.
    • 24 patients underwent surgical resection for metastatic disease, 5 with liver metastases.
    • Multivariate analysis showed that resection, but not the site of metastasis, was a significant predictor of survival.
    • The median survival for patients undergoing resection was 38 months, with a 5-year survival of 39%.
  • Pawlik et al. reported on 16 patients with liver metastases from ocular melanoma who underwent resection.
    • The median time to recurrence was 8.8 months.
    • Compared with patients undergoing liver resection for metastatic cutaneous melanoma:
      • More patients with ocular melanoma experienced recurrent disease in the liver (53% vs. 17%).
      • However, the 5-year survival was significantly better for metastatic ocular melanoma (21% vs. 0%; P = .015).
  • Despite these data, most patients with liver metastases from uveal melanoma are not candidates for complete resection.

Imaging and Patient Selection

  • For optimal selection of patients eligible for liver resection in uveal melanoma, diligent staging is mandatory.
  • Magnetic Resonance Imaging (MRI) might be superior to Fluorodeoxyglucose Positron-Emission Tomography (FDG-PET), as reported in a preliminary study.

Melanoma Metastases to the Pancreas

  • Gastrointestinal metastases of malignant melanoma are common, but isolated disease of the pancreas is rare.
  • Reports indicate that metastases to the pancreas have been described to originate disproportionately from primary ocular melanoma.
  • Surgical experience is limited to small case series.
  • Surgical resection for pancreatic metastases from melanoma is considered a palliative treatment.
    • Generally performed in patients with:
      • Long disease-free intervals.
      • A negative extent-of-disease workup.
      • After a multidisciplinary discussion.
  • Prognosis after resection:
    • Small series suggest a 5-year survival of 50% for R0 resected isolated melanoma metastases.
    • Compared with 9% for patients treated with non-operative management.
    • Data are retrospective and highly selective; thus, a clear benefit of resection cannot be assessed.

Melanoma Metastases to the Liver

  • Metastatic melanoma to the liver can occur, sometimes with an unknown primary site.
  • Melanoma metastases can form polypoid lesions and mimic primary tumors, especially when the history of melanoma is not apparent.
  • On gross appearance, metastatic melanoma in the pancreas or biliary tree may be black if intensely pigmented.
  • Microscopic examination:
    • Tumor cells are not arranged in a particular pattern but display diffuse and noncohesive infiltration.
    • Immunohistochemistry:
      • Positive for melanocytic markers (S100, SOX10, Melan-A, HMB45).
      • Negative labeling for cytokeratin confirms the diagnosis.

Imaging Findings

  • Computed Tomography (CT):
    • Melanoma liver metastases are hypervascular lesions.
    • Multiphasic imaging is advantageous for detecting hypervascular liver metastases.
    • Approximately 14% of melanoma metastases are not seen on portal venous phase images when the arterial phase is not provided.
  • Magnetic Resonance Imaging (MRI):
    • Metastases may be hyperintense on T1-weighted images due to melanin content.
    • On T2-weighted images, they are usually moderately brighter than the liver.
    • Diffusion-weighted imaging (DWI) provides additional signal characteristics.
    • Subtraction imaging can demonstrate a "doughnut sign", indicating peripheral rim enhancement and central hypoenhancement due to necrosis.

Surgical Management

  • Some propose that patients with isolated hepatic melanoma may benefit from surgery when complete resection is possible.
  • Outcomes after resection:
    • Reports suggest improved survival rates in selected patients.
    • Patients with unknown primary melanoma appear to have equivalent or better outcomes than patients with known primaries of a similar stage.

Imaging in Melanoma

Computed Tomography (CT)

  • State-of-the-art CT evaluation may include:
    • Precontrast images.
    • Contrast-enhanced early arterial phase.
    • Late arterial phase.
    • Portal venous phase.
    • Delayed phase.
  • Routine CT is usually performed in the portal venous phase, but hypervascular neoplasms like melanoma require a multiphasic protocol.

Magnetic Resonance Imaging (MRI)

  • MRI has superior contrast resolution, enhancing its sensitivity for liver metastases.
  • Metastatic tumors like melanoma maintain the histologic features seen in their primary location.
  • Melanin demonstrates high signal on T1-weighted images.
  • MRI diffusion sequences may help in distinguishing cystic metastases from other cystic lesions.

Role of FDG-PET

  • FDG-PET scans may be useful, particularly if the patient has a history of colonic, renal, or melanoma primary tumors.
  • MRI might be superior to FDG-PET in staging patients for liver resection in uveal melanoma.

Molecular Aspects

  • Melanomas fall at the upper end of the spectrum in terms of mutational burden.
  • Tumor Mutation Burden (TMB) is positively correlated with increased likelihood of neoantigen production.
  • This potentially results in a beneficial response to checkpoint inhibitor therapies.

Treatment

Role of Targeted Therapy and Immunotherapy

  • Targeted therapy using BRAF or MEK inhibitors and immune checkpoint inhibitors has led to a paradigm shift in the treatment of metastatic melanoma.
  • Median survival in selected patients with metastatic melanoma and a BRAF V600 mutation can be extended to more than 21 months.
  • Combination therapy with dabrafenib and trametinib resulted in an objective tumor response rate of 64%.
  • Systemic therapies have a powerful impact on patients undergoing resection in metastasized melanoma.
  • Effective systemic therapy might render more patients eligible for a curative surgical approach when they present with liver metastases.

Type I Interferons

  • Type I Interferons, including Interferon-α (IFN-α) and Interferon-β (IFN-β), are crucial in the immune response.
  • IFN-α has been used clinically as an adjuvant therapy for melanoma.
  • They inhibit cellular proliferation and enhance immune responses against tumor cells.

Conclusion

  • Melanoma metastases to the pancreas, liver, and biliary tree are rare but clinically significant.
  • Surgical resection may offer survival benefits in selected patients with isolated metastases and long disease-free intervals.
  • Patient selection is crucial, requiring diligent staging and multidisciplinary discussions.
  • Targeted therapies and immunotherapies have improved survival and may increase eligibility for surgical intervention.
  • A multidisciplinary approach is essential for optimizing patient outcomes in melanoma involving HPB diseases.