11/13/24, 7\:24 PM Guide | Nasopharyngeal swab

Nasopharyngeal swab

Table of contents

Background

This guide provides an overview of performing a nasopharyngeal swab for the purposes of viral sample collection (e.g. COVID-
19) in an OSCE setting. It does not include details on the appropriate personal protective equipment (PPE) required, which is
pathogen dependent (see our guide to PPE).
It should be noted that this guide only provides a general approach to sample collection and the details regarding storage
medium and transport vary depending on the pathogen being tested for. You should always consult your local guidelines
before taking a sample from a patient.

Gather equipment

Gather the relevant equipment for the procedure\:
Gloves and other appropriate PPE.
Flexible plastic nasopharyngeal swab.
Specimen tube containing universal transport medium.
Nasopharyngeal swab tips for the purposes of viral sample collection must be synthetic. Cotton or calcium alginate tipped
swabs can kill viral particles and/or interfere with PCR testing.
Only swabs with

Introduction

Wash your hands and don PPE.
Introduce yourself to the patient including your name and role.
Con
Brie
be uncomfortable and will likely elicit a gag re
Gain consent to proceed with performing a nasopharyngeal swab.
Ask the patient if they have any pain before continuing with the clinical procedure.
Position the patient sitting comfortably on a chair or bed.

Nasopharyngeal swab

1. With the patient seated, ask them to
passages.
2. Carefully open the swab packaging, only handling the swab above the breakpoint.
3. Measure the distance from the corner of the nose to the front of the ear and insert the shaft ONLY half this length. In adults,
this distance is usually around 4 cm.
4. Tilt the patient's head back 70° to straighten the passage between the nose and the nasopharynx.
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5. Avoiding contact with external nasal skin, insert the swab along the medial part of the septum, along the base of the nose
(gently rotating the swab may be helpful to aid insertion). If resistance is encountered on one side, try the alternative nostril as
the patient may have a deviated nasal septum.
6. Once the swab is fully inserted, leave it in place for several seconds to absorb the nasopharyngeal secretions.
7. Slowly remove the nasopharyngeal swab whilst rotating it.
8. Insert the nasopharyngeal swab into a tube of universal transport medium, ensuring that the swab tip is fully submerged into
the liquid of the tube.
9. Snap o
submerged in the tube's liquid.
10. Label the sample with the patient's name, date of birth and unique identi
11. Appropriately package the specimen for transport to the lab.
12. Arrange for transport of the specimen within an appropriate time frame.
Nasopharyngeal swab

To complete the procedure...

Explain to the patient that the procedure is now complete.
Thank the patient for their time.
O
Dispose of PPE appropriately and wash your hands.
Document the details of the procedure in the patient's notes\:
Your personal details including your name, job role and GMC number.
The date and time the procedure was performed.
Con
The indication for performing a nasopharyngeal swab.
Any complications that occurred during the procedure.
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References

1. Centers for Disease Control (CDC) and Prevention. InLINK]
2. World Health Organisation. WHO guidelines for the collection of human specimens for laboratory diagnosis of avian in
infection. Published 12th January 2005. Available from\: [LINK]
Source\: geekymedics.com
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